Comparative transcriptomics reveals different strategies of Trichodermamycoparasitism
Lea Atanasova, Stephane Le Crom, Sabine Gruber, Fanny Coulpier, Verena Seidl-Seiboth, Christian P Kubicek, Irina S Druzhinina
BMC Genomics·2013·257 citations
<jats:title>Abstract</jats:title>
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<jats:title>Background</jats:title>
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<jats:italic>Trichoderma</jats:italic> is a genus of mycotrophic filamentous fungi (teleomorph <jats:italic>Hypocrea</jats:italic>) which possess a bright variety of biotrophic and saprotrophic lifestyles. The ability to parasitize and/or kill other fungi (mycoparasitism) is used in plant protection against soil-borne fungal diseases (biological control, or biocontrol). To investigate mechanisms of mycoparasitism, we compared the transcriptional responses of cosmopolitan opportunistic species and powerful biocontrol agents <jats:italic>Trichoderma atroviride</jats:italic> and <jats:italic>T. virens</jats:italic> with tropical ecologically restricted species <jats:italic>T. reesei</jats:italic> during confrontations with a plant pathogenic fungus <jats:italic>Rhizoctonia solani</jats:italic>.</jats:p>
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<jats:title>Results</jats:title>
<jats:p>The three <jats:italic>Trichoderma</jats:italic> spp. exhibited a strikingly different transcriptomic response already before physical contact with alien hyphae. <jats:italic>T. atroviride</jats:italic> expressed an array of genes involved in production of secondary metabolites, GH16 ß-glucanases, various proteases and small secreted cysteine rich proteins. <jats:italic>T. virens</jats:italic>, on the other hand, expressed mainly the genes for biosynthesis of gliotoxin, respective precursors and also glutathione, which is necessary for gliotoxin biosynthesis. In contrast, <jats:italic>T. reesei</jats:italic> increased the expression of genes encoding cellulases and hemicellulases, and of the genes involved in solute transport. The majority of differentially regulated genes were orthologues present in all three species or both in <jats:italic>T. atroviride</jats:italic> and <jats:italic>T. virens</jats:italic>, indicating that the regulation of expression of these genes is different in the three <jats:italic>Trichoderma</jats:italic> spp. The genes expressed in all three fungi exhibited a nonrandom genomic distribution, indicating a possibility for their regulation via chromatin modification.</jats:p>
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<jats:title>Conclusion</jats:title>
<jats:p>This genome-wide expression study demonstrates that the initial <jats:italic>Trichoderma</jats:italic> mycotrophy has differentiated into several alternative ecological strategies ranging from parasitism to predation and saprotrophy. It provides first insights into the mechanisms of interactions between <jats:italic>Trichoderma</jats:italic> and other fungi that may be exploited for further development of biofungicides.</jats:p>
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