Highest Rated Papers
Chikungunya Virus: Emerging Targets and New Opportunities for Medicinal Chemistry
Adel A. Rashad, Suresh Mahalingam, Paul A. Keller
Journal of Medicinal Chemistry·2013·76 citations
No abstract available
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View paper →Cryo-EM structure of the mature and infective Mayaro virus at 4.4 Å resolution reveals features of arthritogenic alphaviruses
Helder V. Ribeiro-Filho, Lais D. Coimbra, Alexandre Cassago, Rebeca P. F. Rocha, João Victor da Silva Guerra, Rafael de Felicio, Carolina Moretto Carnieli, Luiza Leme, Antonio Cláudio Padilha, Adriana F. Paes Leme, Daniela B. B. Trivella, Rodrigo Villares Portugal, Paulo Sérgio Lopes-de-Oliveira, Rafael Elias Marques
Nature Communications·2021·49 citations
<jats:title>Abstract</jats:title>
<jats:p>Mayaro virus (MAYV) is an emerging arbovirus of the Americas that may cause a debilitating arthritogenic disease. The biology of MAYV is not fully understood and largely inferred from related arthritogenic alphaviruses. Here, we present the structure of MAYV at 4.4 Å resolution, obtained from a preparation of mature, infective virions. MAYV presents typical alphavirus features and organization. Interactions between viral proteins that lead to particle formation are described together with a hydrophobic pocket formed between E1 and E2 spike proteins and conformational epitopes specific of MAYV. We also describe MAYV glycosylation residues in E1 and E2 that may affect MXRA8 host receptor binding, and a molecular “handshake” between MAYV spikes formed by N262 glycosylation in adjacent E2 proteins. The structure of MAYV is suggestive of structural and functional complexity among alphaviruses, which may be targeted for specificity or antiviral activity.</jats:p>
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View paper →Molecular epidemiological investigation of Mayaro virus in febrile patients from Goiania City, 2017–2018
Elisângela de Paula Silveira-Lacerda, Alice Laschuk Herlinger, Amilcar Tanuri, Giovanni Rezza, Carlos Eduardo Anunciação, Juliana Pires Ribeiro, Isabele Pereira Tannous, Gabrielly Regis Abrantes, Elisângela Gomes da Silva, Kauana Ferreira Arruda, Adriano Roberto Vieira de Sousa, Filipe Romero Rebello Moreira, Renato Santana Aguiar, Jordana Faria Corrêa, Marina Machado dos Santos, Hugo Delleon Silva, Marco Tulio A. Garcia-Zapata, Natália Santana do Nascimento, Mariane Talon de Menezes, Richard Araujo Maia, Orlando Ferreira C., Rafael Barbosa, Rodrigo Brindeiro, Clever Cardoso, Sandra Maria Brunini
Infection, Genetics and Evolution·2021·15 citations
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View paper →Will Mayaro virus be responsible for the next outbreak of an arthropod-borne virus in Brazil?
Danillo Lucas Alves Esposito, Benedito Antonio Lopes da Fonseca
The Brazilian Journal of Infectious Diseases·2017·88 citations
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View paper →SAM 3D Body: Robust Full-Body Human Mesh Recovery | Research - AI at Meta
Xitong Yang, Devansh Kukreja, Don Pinkus, Anushka Sagar, Taosha Fan, Jinhyung Park, Soyong Shin, Jinkun Cao, Jiawei Liu, Nicolas Ugrinovic, Matt Feiszli, Jitendra Malik, Piotr Dollar, Kris Kitani
ai.meta.com
We introduce SAM 3D Body (3DB), a promptable model for single-image full-body 3D human mesh recovery (HMR) that demonstrates state-of-the-art...
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View paper →Variation in foraging strategies over a large spatial scale reduces parent–offspring conflict in Manx shearwaters
Saskia Wischnewski, Gavin E. Arneill, Ashley W. Bennison, Eileen Dillane, Timothée A. Poupart, Camilla A. Hinde, Mark J. Jessopp, John L. Quinn
Animal Behaviour·2019·13 citations
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View paper →Epidemiology in the context of white supremacy: critical questions to align the discipline with health equity
Jessie Seiler, Nadia N. Abuelezam
Critical Public Health·2025
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View paper →Antiviral Strategies against Arthritogenic Alphaviruses
Rana Abdelnabi, Leen Delang
Microorganisms·2020·25 citations
<jats:p>Alphaviruses are members of the Togaviridae family that are mainly transmitted by arthropods such as mosquitoes. In the last decades, several alphaviruses have re-emerged, causing outbreaks worldwide. One example is the re-emergence of chikungunya virus (CHIKV) in 2004, which caused massive epidemics in the Indian Ocean region after which the virus dramatically spread to the Americas in late 2013. Besides CHIKV, other alphaviruses, such as the Ross River virus (RRV), Mayaro virus (MAYV), and Venezuelan equine encephalitis virus (VEEV), have emerged and have become a serious public health concern in recent years. Infections with the Old World alphaviruses (e.g., CHIKV, RRV) are primarily associated with polyarthritis and myalgia that can persist for months to years. On the other hand, New World alphaviruses such as VEEV cause mainly neurological disease. Despite the worldwide (re-)emergence of these viruses, there are no antivirals or vaccines available for the treatment or prevention of infections with alphaviruses. It is therefore of utmost importance to develop antiviral strategies against these viruses. We here provided an overview of the reported antiviral strategies against arthritogenic alphaviruses. In addition, we highlighted the future perspectives for the development and the proper use of such antivirals.</jats:p>
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View paper →Mayaro: an emerging viral threat?
Yeny Acosta-Ampudia, Diana M. Monsalve, Yhojan Rodríguez, Yovana Pacheco, Juan-Manuel Anaya, Carolina Ramírez-Santana
Emerging Microbes & Infections·2018·97 citations
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View paper →A silent public health threat: emergence of Mayaro virus and co-infection with Dengue in Peru
Miguel Angel Aguilar-Luis, Juana del Valle-Mendoza, Isabel Sandoval, Wilmer Silva-Caso, Fernando Mazulis, Hugo Carrillo-Ng, Yordi Tarazona-Castro, Johanna Martins-Luna, Ronald Aquino-Ortega, Isaac Peña-Tuesta, Angela Cornejo-Tapia, Luis J. del Valle
BMC Research Notes·2021·23 citations
<jats:title>Abstract</jats:title>
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<jats:title>Objective</jats:title>
<jats:p>To describe frequency and clinical characteristics of MAYV infection in Piura, as well as the association of this pathogen with DENV.</jats:p>
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<jats:title>Results</jats:title>
<jats:p>A total of 86/496 (17.3%) cases of MAYV were detected, of which 54 were MAYV mono-infection and 32 were co-infection with DENV, accounting for 10.9% and 6.4%, respectively. When evaluating monoinfection by MAYV the main groups were 18–39 and 40–59 years old, with 25.9% and 20.4% respectively. Co-infections were more common in the age group 18–39 and those > 60 years old, with 34.4% and 21.9%, respectively. The most frequent clinical presentation were headaches (94.4%, 51/54) followed by arthralgias (77.8%, 42/54). During the 8-month study period the most cases were identified in the months of May (29.1%) and June (50.0%).</jats:p>
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View paper →Mayaro Virus: The State-of-the-Art for Antiviral Drug Development
Ana Paula Andreolla, Alessandra Abel Borges, Juliano Bordignon, Claudia Nunes Duarte dos Santos
Viruses·2022·12 citations
<jats:p>Mayaro virus is an emerging arbovirus that causes nonspecific febrile illness or arthralgia syndromes similar to the Chikungunya virus, a virus closely related from the Togaviridae family. MAYV outbreaks occur more frequently in the northern and central-western states of Brazil; however, in recent years, virus circulation has been spreading to other regions. Due to the undifferentiated initial clinical symptoms between MAYV and other endemic pathogenic arboviruses with geographic overlapping, identification of patients infected by MAYV might be underreported. Additionally, the lack of specific prophylactic approaches or antiviral drugs limits the pharmacological management of patients to treat symptoms like pain and inflammation, as is the case with most pathogenic alphaviruses. In this context, this review aims to present the state-of-the-art regarding the screening and development of compounds/molecules which may present anti-MAYV activity and infection inhibition.</jats:p>
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View paper →A Chikungunya Virus <i>trans</i> -Replicase System Reveals the Importance of Delayed Nonstructural Polyprotein Processing for Efficient Replication Complex Formation in Mosquito Cells
Koen Bartholomeeusen, Age Utt, Sandra Coppens, Kai Rausalu, Katleen Vereecken, Kevin K. Ariën, Andres Merits
Journal of Virology·2018·41 citations
<jats:title>ABSTRACT</jats:title>
<jats:p>
Chikungunya virus (CHIKV) is a medically important alphavirus that is transmitted by
<jats:named-content content-type="genus-species">Aedes aegypti</jats:named-content>
and
<jats:named-content content-type="genus-species">Aedes albopictus</jats:named-content>
mosquitoes. The viral replicase complex consists of four nonstructural proteins (nsPs) expressed as a polyprotein precursor and encompasses all enzymatic activities required for viral RNA replication. nsPs interact with host components of which most are still poorly understood, especially in mosquitos. A CHIKV
<jats:italic>trans</jats:italic>
-replicase system that allows the uncoupling of RNA replication and nsP expression was adapted to mosquito cells and subsequently used for analysis of universal and host-specific effects of 17 different nonstructural polyprotein (ns-polyprotein) mutations. It was found that mutations blocking nsP enzymatic activities as well as insertions of enhanced green fluorescent protein (EGFP) into different nsPs had similar effects on
<jats:italic>trans</jats:italic>
-replicase activity regardless of the host (i.e., mammalian or mosquito). Mutations that slow down or accelerate ns-polyprotein processing generally had no effect or reduced
<jats:italic>trans</jats:italic>
-replicase activity in mammalian cells, while in mosquito cells most of them increased
<jats:italic>trans</jats:italic>
-replicase activity prominently. Increased RNA replication in mosquito cells was counteracted by an antiviral RNA interference (RNAi) response. Substitution of the W258 residue in the membrane binding peptide of nsP1 resulted in a temperature-sensitive defect, in the context of both the
<jats:italic>trans</jats:italic>
-replicase and infectious CHIKV. The defect was compensated for by secondary mutations selected during passaging of mutant CHIKV. These findings demonstrate the value of alphavirus
<jats:italic>trans</jats:italic>
-replicase systems for studies of viral RNA replication and virus-host interactions.
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<jats:p>
<jats:bold>IMPORTANCE</jats:bold>
Chikungunya virus is an important mosquito-transmitted human pathogen. This virus actively replicates in mosquitoes, but the underlying molecular mechanisms and interactions of viral and host components are poorly understood. This is partly due to the lack of reliable systems for functional analysis of viral nonstructural polyproteins (ns-polyproteins) and nonstructural proteins (nsPs) in mosquito cells. Adaption of a CHIKV
<jats:italic>trans</jats:italic>
-replicase system allowed study of the effects of mutations in the ns-polyprotein on RNA replication in cells derived from mammalian and mosquito hosts. We found that a slowdown of ns-polyprotein processing facilitates replication complex formation and/or functioning in mosquito cells and that this process is antagonized by the natural RNAi defense system present in mosquito cells. The mosquito-adapted CHIKV
<jats:italic>trans</jats:italic>
-replicase system represents a valuable tool to study alphavirus-mosquito interactions at the molecular level and to develop advanced antiviral strategies.
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