Lowest Rated Papers
Pequenas reflexões #1: Pensar crítico x Antiintelectualismo
Fashionismo | Thereza Chammas
Substack·2025
Pequenas reflexões, grandes pensamentos!
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View paper →Expanding Our View of Drosophila Centrioles
Emma Burns, Anastasia Amoiroglou, Carey J. Fagerstrom, John M. Ryniawec, LingSze Lee, Rose K. Runyan, Leah F. Rosin, Gregory C. Rogers, Nasser Rusan
Journal of Cell Science·2025
A significant challenge in Drosophila centriole biology is its small size. Advanced super-resolution techniques have provided valuable insights, but require specialized equipment and can be difficult to implement in tissues. Expansion Microscopy (ExM) offers an accessible alternative, yet its application in Drosophila centriole research has been sparse. We provide an ExM protocol for cultured S2 cells and fly tissues that revealed new insights into pro-centriole biology. In S2 cells we document overduplication in the form of the classic "rosettes", while in spermatids we uncover an unexpected movement of the pro-centriole-like structure (PCL). ExM has also refined existing molecular models. In S2 cells we document the distal tip protein Cep97 as a ring, which clarifies its role in capping the growing centriole. In spermatids, we spatially segregated the inner nuclear membrane protein Spag4 and the cytoplasmic protein Yuri, which led to the new hypothesis that they play independent roles at the centriole-nucleus contact site. Finally, we show that our ExM protocol is a hypothesis-generator and discovery tool applicable beyond Drosophila centrioles by imaging synaptonemal complexes in the Plodia interpunctella moth.
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View paper →Spectral phasor plot for hyperspectral imaging of acridine orange photophysics within the cell
Diaz, M., Zolessi, F. R., Malacrida, L. S.
bioRxiv·2023
Acridine Orange (AO) exhibits complex photophysical properties that have been utilized to study its interactions with RNA and DNA. Traditionally, AO has been used to stain cells, distinguishing nucleus and cytoplasm based on differences in DNA and RNA composition. Its labeling behavior varies depending on factors such as concentration and pH. AO fluorescence is rich and complex; therefore, acquiring the full spectrum or the fluorescence lifetime is beneficial for understanding its intricate photophysics. Nevertheless, handling 4D datasets can be challenging and often relies on sophisticated or highly parameterized models for their analysis. Model-free approaches, such as spectral phasor (SP) analysis for hyperspectral imaging (HSI), are well suited to navigating these analytical challenges. Using SP principles of linear combination and reciprocity, we identified spectral fingerprints of RNA and DNA in in vitro experiments that remain consistent in both live and fixed cells. Interestingly, SP analysis of AO fluorescence in live cells revealed a third spectral component at 640 nm. The linear properties of SP analysis enabled the quantification of AO bound to RNA, DNA, and the third component. Co-labeling with AO and LysoTracker suggested that some of these subcellular structures are associated with acidic compartments. Moreover, SP analysis distinguished AO self-interactions from its RNA binding in cytoplasmic compartments. This study establishes SP analysis as a robust tool for hyperspectral imaging of AO, thereby enhancing our understanding of its intricate photophysics and unlocking new opportunities for advanced in vivo cell imaging applications.
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View paper →The Past 110 Years: Historical Data on the Underrepresentation of Women in Philosophy Journals
Nicole Hassoun, Sherri Conklin, Michael Nekrasov, Jevin West
Ethics·2022·14 citations
This article provides the first large-scale, longitudinal study examining publication rates by gender in philosophy journals. We find that from 1900 to 1990 the proportion of women authorships in philosophy increased, but it has plateaued since the 1990s (unlike in other disciplines). Top Philosophy journals publish the lowest proportion of women, and anonymous review does not increase the proportion publishing in these journals (though it does in other journals). Value Theory journals do not publish articles by women in proportion to their presence in the subdiscipline. Although the proportion of women authorships in philosophy has increased over time, measurable disparities persist.
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View paper →Simone de Beauvoir and Betty Friedan: The Politics of Omission
Sandra Dijkstra
Feminist Studies·1980·7 citations
No abstract available
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View paper →Wollstonecraft's Philosophical Impact on Nineteenth‐Century American Women's Rights Advocates
Eileen Hunt Botting, Christine Carey
American Journal of Political Science·2004·10 citations
This article challenges the thesis that the publication of William Godwin's scandalous Memoirs of the Author of A Vindication of the Rights of Woman in 1798 minimized the philosophical impact of Mary Wollstonecraft's 1792 work the Rights of Woman in nineteenth-century American political thought. Instead, we demonstrate that leading nineteenth-century American women's rights advocates—Hannah Mather Crocker, Lucretia Mott, Sarah Grimké, Margaret Fuller, Elizabeth Cady Stanton, and Susan B. Anthony—understood themselves to be in a critical, philosophical dialogue with the text of the Rights of Woman, and in some cases, the Memoirs, and defined their own, distinctive philosophies of sex equality partly within this context.
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View paper →Do Artifacts Have Politics?
Langdon Winner
MIT Press·1980
In controversies about technology and society, there is no idea more provocative than the notion that
technical things have political qualities. At issue is the claim that the machines, structures, and systems of modern material culture can be accurately judged not only for their contributions of efficiency and productivity, not merely for their positive and negative environmental side effects, but also for the ways in which they can embody specific forms of power and authority. Since ideas of this kind have a persistent and troubling presence in discussions about the meaning of technology, they deserve explicit attention.1
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View paper →Culture meets Artificial Intelligence
Kursat Ozenc
Medium·2018
6 Reflections on Stanford’s Culturally Relevant AI Summit
This past October, we attended the Culturally Relevant AI Summit at Stanford, organized by Media X. The day comprised intriguing lightning talks and discussions. Here’re our six reflections from the AI meets Culture.
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View paper →CD4+ T
Jean-Philippe Herbeuval, Jean-Charles Grivel, Adriano Boasso, Andrew W Hardy, Claire Chougnet, Matthew J Dolan, Hideo Yagita, Jeffrey D Lifson, Gene M Shearer
Blood·2005
It has been proposed that direct and indirect mechanisms contribute to the unresolved issue of CD4(+) T-cell depletion that results from HIV-1 infection. We recently reported that plasma levels of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) are elevated in HIV-1-infected patients and that they correlate with viral load. The present study investigates the expression of TRAIL death receptor 5 (DR5) in the peripheral-blood mononuclear cells (PBMCs) of HIV-1-infected patients and its role in CD4(+) T-cell death. DR5 expression was elevated and associated with the apoptotic marker annexin V. Apoptosis was reduced in CD4(+) T cells when cultured with anti-DR5 antibody. CD4(+), but not CD8(+), T cells from uninfected donors expressed TRAIL, DR5, and activated caspase-3 when cultured with infectious or noninfectious HIV-1, resulting in preferential apoptosis of CD4(+) T cells. TRAIL, caspase-3 expression, and apoptosis were type 1 interferon (IFN) dependent. Induction of apoptosis and DR5 expression required glycoprotein 120 (gp120)-CD4 interaction. Finally, we analyzed DR5 expression by CD4(+) T cells in highly active antiretroviral therapy (HAART)-treated patients. The decreased viral loads and increased CD4 counts of HAART-responsive patients were associated with a decrease in DR5 mRNA expression by CD4(+) T lymphocytes. We propose a novel model in which a type 1 IFN-regulated TRAIL /DR5 mechanism induces apoptosis of HIV-1-exposed CD4(+) T cells.
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View paper →The size of the plasmacytoid dendritic cell compartment is a multigenic trait dominated by a locus on mouse chromosome 7.
Adam-Nicolas Pelletier, Fanny Guimont-Desrochers, Michelle P Ashton, Thomas C Brodnicki, Sylvie Lesage
Journal of immunology (Baltimore, Md. : 1950)·2012
Plasmacytoid dendritic cells (pDC) compose one of the many distinct dendritic cell subsets. The primary function of pDC is to potently produce type 1 IFNs upon stimulation, which is highly relevant in antiviral responses. Consequently, the ability to manipulate the size of the pDC compartment in vivo may increase the capacity to clear viral infections. In an attempt to identify genetic loci affecting the size of the pDC compartment, defined by both the proportion and absolute number of pDC, we undertook an unbiased genetic approach. Linkage analysis using inbred mouse strains identified a locus on chromosome 7 (Pdcc1) significantly linked to both the proportion and the absolute number of pDC in the spleen. Moreover, loci on either chromosome 11 (Pdcc2) or 9 (Pdcc3) modified the effect of Pdcc1 on chromosome 7 for the proportion and absolute number of pDC, respectively. Further analysis using mice congenic for chromosome 7 confirmed Pdcc1, demonstrating that variation within this genetic interval can regulate the size of the pDC compartment. Finally, mixed bone marrow chimera experiments showed that both the proportion and the absolute number of pDC are regulated by cell-intrinsic hematopoietic factors. Our findings highlight the multigenic regulation of the size of the pDC compartment and will facilitate the identification of genes linked to this trait.
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View paper →Endothelial cells are central orchestrators of cytokine amplification during influenza virus infection.
John R Teijaro, Kevin B Walsh, Stuart Cahalan, Daniel M Fremgen, Edward Roberts, Fiona Scott, Esther Martinborough, Robert Peach, Michael B A Oldstone, Hugh Rosen
Cell·2011
Cytokine storm during viral infection is a prospective predictor of morbidity and mortality, yet the cellular sources remain undefined. Here, using genetic and chemical tools to probe functions of the S1P(1) receptor, we elucidate cellular and signaling mechanisms that are important in initiating cytokine storm. Whereas S1P(1) receptor is expressed on endothelial cells and lymphocytes within lung tissue, S1P(1) agonism suppresses cytokines and innate immune cell recruitment in wild-type and lymphocyte-deficient mice, identifying endothelial cells as central regulators of cytokine storm. Furthermore, our data reveal immune cell infiltration and cytokine production as distinct events that are both orchestrated by endothelial cells. Moreover, we demonstrate that suppression of early innate immune responses through S1P(1) signaling results in reduced mortality during infection with a human pathogenic strain of influenza virus. Modulation of endothelium with a specific agonist suggests that diseases in which amplification of cytokine storm is a significant pathological component could be chemically tractable.
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View paper →Two Concerns about the Medicalization of Love
MARTIN O’REILLY
Cambridge Quarterly of Healthcare Ethics·2015·1 citations
No abstract available
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